![]() The inoculated plates were incubated for 15 days at 25 ☌ and 50–60% RH. 2008) was propagated on Czapek’s Dox agar medium. anisopliaeĪ local strain of the fungus originally isolated from a naturally infected mole cricket, Gryllotalpa gryllotalpa L. The third (L 3) and fifth (L 5) larval instars were used for testing the efficacy of M. ![]() The diet amount in each cell is quite enough for the larva to complete development and pupate. One larva was confined in each cell on the diet and the structure was covered with a plastic plate perforated with four fine openings over each cell for aeration. A plastic grid of 80 cubic cells (2 × 2 × 3 cm) was pressed on the diet tray. The prepared diet was poured before solidifying into trays (20 × 30 cm) in a layer of 2 cm in thickness. ![]() littoralis was reared for many generations on the semi-synthetic diet of Shorey and Hale ( 1965) at 25 ± 1 ☌ and 50–60% RH at the Centre of Biological Control, Faculty of Agriculture, Cairo University, Egypt. littoralis, reared on a semi-synthetic diet under laboratory conditions.Ī laboratory strain of S. anisopliae against third and fifth larval instars of the cotton leafworm, S. The present study aimed to test the efficacy of a local isolate of M. Petlamul and Prasertsan ( 2012) related such differences to germination rate, conidia production, radial growth, and enzyme activity. The different isolates of EPFs differ in their efficacy against targeted insect hosts. They infect insects by penetrating the body, using extracellular cuticle-hydrolyzing enzymes of lipases, proteases, and chitinases (St. EPFs were used worldwide for controlling different stages of many insect pests (Liu and Li 2004). Thus, search became urgent for new alternative and environmentally safe biocide agents such as the entomopathogenic fungi (EPF) (Anand et al. 2016), environmental pollution, and negative impact on non-target insect species like pollinators, insect parasitoids, and predators. The continuous use of chemical pesticides against this pest resulted in resistant strains to most applied insecticides (Shad et al. Control of this pest was always practiced by applying chemical insecticides. In Egypt, it is considered a serious pest on cotton, corn, vegetables, and medicinal and aromatic plants (Amer et al. The cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), is a polyphagous insect pest attacking a wide range of host plants including vegetables and ornamental plants (Rao et al.
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